Exogenous Polyamines Influence In Vitro Microbial Adhesion to Human Mucus according to the Age of Mucus Donor

Adhesion to intestinal mucus is the first step for microbiota colonization in early life. Polyamines are polycations with important physiological functions in both procaryotic and eucaryotic cells. However, their role in intestinal mucus adhesion is not known. The objective of the present study was to evaluate whether exogenous polyamines (putrescine, spermidine, spermine, and their combination) would alter the adhesive properties of Lacticaseibacillus rhamnosus GG (LGG), Bifidobacterium animalis subs. lactis Bb12, Cronobacter sakazakii, and Escherichia coli. Human intestinal mucus was isolated from healthy infants (0–6-month-old and 6–12-month-old) and healthy adults (25–52 years old). Spermidine significantly increased Bb12 adhesion (p