Transmission of grapevine virus H by the vine mealybug (Planococcus ficus Sign.)

Veliki broj virusa može zaraziti jednu od najuzgajanijih kultura u svijetu, a to je vinova loza (Vitis vinifera L.). Među njima je i novootkriveni H-virus vinove loze (grapevine virus H, GVH) koji pripada rodu Vitivirus. Prijenos pojedinih virusa vinove loze, uz zaraženi sadni materijal, moguć je i pomoću štitastih ušiju. U laboratorijskim uvjetima, pokusi prijenosa GVH pomoću lozine štitaste uši (Planococcus ficus Sign.) na sadnice vinove loze sorte Žlahtina bili su uspješni, što je i dokazano njihovim testiranjem metodom lančane reakcije polimerazom nakon obrnutog prepisivanja (RT-PCR) u stvarnom vremenu (real-time). Od 19 biljaka vinove loze, kod dvije je potvrđena zaraza. Isto je potvrđeno i pomoću konvecionalnog RT-PCR-a, a njegovi produkti su vizualizirani provedbom horizontalne gel elektroforeze. Ostatak test biljaka/korova uključenih u istraživanje (korovi Paper rhoeas, Abuthilon theophrasti, Amarathus retroflexus, Amrosia artemisifolia, dvije zeljaste test biljke Nicotiana benthamiana i Chenopodium murale) pokazale su se negativnim u provedenim molekularnim testovima.Many different viruses have been reported to infect one of the most commonly grow plants, grapevine ( Vitis vinifera L.). One of these is the recently discovered grapevine virus H (GVH), which belongs to the genus Vitivirus. Transmission of some grapevine viruses occurs through mealybugs, in addition to the use of contaminated planting material. Under experimental conditions, we obtained successful transmission of GVH by vine mealybug (Planococcus ficus Sign.) to seedlings of grapevine cv. Žlahtina, which was confirmed by real-time RT-PCR. Two out of 19 vine seedlings tested positive. The same was confirmed by conventional one-step reverse transcription polymerase chain reaction (RT-PCR) and the products were visualized by horizontal gel electrophoresis. Other plants used in the study (weed species Paper rhoeas, Abuthilon theophrasti, Amarathus retroflexus, Amrosia artemisifolia; and two herbaceous test plants Nicotiana benthamiana and Chenopodium murale) tested negative using the real-time RT-PCR