The influence of osmotic stress on the germination of the seeds of dalmatian pyrethrum, common sage and winter savory

Dalmatinski buhač (Tanacetum cinerariifolium (Trevis.) Sch. Bip.), ljekovita kadulja (Salvia officinalis L.) i primorski vrisak (Satureja montana L.) tipični su predstavnici mediteranske klime. Posljedice klimatskih promjena najviše će se odraziti na područje Mediterana, gdje se očekuju razdoblja jačih suša zbog čega će mnoge biljne vrste biti suočene s nestašicom vode. Cilj ovog diplomskog rada bio je utvrditi utjecaj vodnog deficita (različitog intenziteta suše) na klijavost sjemena navedenih vrsta. Sjeme je podvrgnuto trima razinama osmotskog stresa (-0,2, -0,5, -1,0 MPa), primjenom različitih koncentracija otopine polietilen glikola (PEG 8000). Visoke razine suše (-0,5 i -1,0 MPa) inhibirale su klijanje sjemena ispitivanih vrsta. U odnosu na kontrolni tretman, ukupna klijavost sjemena istraživanih vrsta bila je niža pri osmotskom stresu od -0,2 MPa (7,5 % vs. 18,75 % kod dalmatinskog buhača; 8,75 % vs. 11,25 % kod ljekovite kadulje; 48,75 % vs. 82,5 % kod primorskog vriska). Prosječno vrijeme klijanja kod primorskog vriska i ljekovite kadulje bilo je duže pri -0,2 MPa (6,28 i 11 dana), dok su vrijednosti indeksa klijavosti kod svih istraživanih vrsta bile veće kod kontrolnog tretmana.Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.), common sage (Salvia officinalis L.) and winter savory (Satureja montana L.) are typical representatives of the Mediterranean climate. The consequences of climate change will be particularly noticeable in the Mediterranean region, where more severe periods of drought are to be expected, because of which many plant species will be confronted with water shortages. The aim of this work was to determine the influence of water deficits (varying intensity of drought) on the germination of seeds of the species mentioned. Seeds were subjected to three levels of osmotic stress (-0.2, -0.5 and -1.0 MPa) using different concentrations of polyethylene glycol solution (PEG 8000). High drought levels (-0.5 and -1.0 MPa) inhibited germination of the tested species. Compared to the control treatment, the total germination of the tested seeds was lower at an osmotic stress of -0.2 MPa (7.5% vs. 18.75% for dalmatian pyrethrum; 8.75% vs. 11.25% for common sage; 48.75% vs. 82.5% for winter savory). The average germination time for winter savory and common sage was longer at -0.2 MPa (6.28 and 11 days), while the germination index values for all studied species were higher in the control treatment