DNA release from cationic liposome/DNA complexes by anionic lipids

The authors found that recently developed multicomponent cationic liposome DNA complexes lipoplexes exhibit higher transfection efficiency with respect to usually employed binary lipoplexes in NIH 3T3 and A17 cell lines. Interaction of lipoplexes with anionic liposomes (model of cellular membranes) was investigated by synchrotron small angle x-ray diffraction. The authors used one-dimensional DNA packing density to estimate the molar fraction of DNA released from lipoplexes by anionic lipids