Polyclonal antibodies immunoprecipitate Ago1 and Ago2 exclusively.

(A) Domain map of Ago1 and Ago2. (B) The guinea pig (GP) antiserum against Ago1 is able to immunoprecipitate Ago1 as detected by a commercial rabbit (RB) anti-Ago1 antibody [Abcam]. (C) The guinea pig (GP) antiserum against Ago2 is able to immunoprecipitate Ago2 as detected by a commercial rabbit (RB) anti-Ago2 antibody [Abcam]. (D) Small RNAs immunoprecipitated with Ago1 or Ago2 antisera from whole wildtype male lysate were sequenced and mapped to known Drosophila miRNAs and transposons (n = 2). miRNAs (black circles) and siRNAs (gray triangles) are shown as percent of total mapped reads. Several miRNAs that have been previously observed to be highly abundant in Ago2 RISC are noted. Representative plot.