Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in <i>Xenopus tropicalis snai2:eGFP</i> line

AbstractTransgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization. Using I-SceI, we previously developed a transgenic Xenopus tropicalissnai2snai2:eGFPXenopus tropicali