Histological and Chronological Study of Myostatin (MSTN) Role on Muscles Growth in Domestic Broiler Chicken

Chicken meat have a low-cholesterol levels, low-fat with a high unsaturation amount of fatty acids, which moreover be considered as functional foods, which provide bioactive ingredients with advantageous effects on human health like vitamins and antioxidants. Therefore, this project was designed to focusing on the relationship between increased the growth curve of skeletal muscles and the role of Myostatin (MSTN) in local domestic broiler chicken. The 50 birds of male domestic broiler chicken were collected from commercial field of Al -Kut, and according ages, the birds were divided into five groups (1day, 8 day, 15 day, 22day, and 30day). The samples of skeletal muscles were taken from the breast and leg areas. All samples were exposed to histological investigation by H&E stains, as well as immunohistochemical technique by a primary and secondary antibody of MSTN through paraffin-embedded sections. Histological results apparent a general structure of skeletal muscles at different ages through synchronous growth development of chicken.  Immunohistochemical investigation appeared the different attribution of MSTN reactive in the skeletal muscle sections at sequence ages of chicken. Surface blots analysis of MSTN in skeletal muscle at the five experimental stages was (3.096, 10.049, 12.927, 18.453, 22.744) respectively.&nbsp