Characterization of fennel honey by cromatografic tehnique (GcGC-MS) and spectroscopy (FTIR-ATK) : master thesis

U ovom radu analiziran je kemijski sastav meda od komorača. Hlapljivi spojevi izolirani su mikroekstrakcijom vršnih para na krutoj fazi (HS-SPME) s dva različita vlakna (sivo vlakno i plavo vlakno) i ultrazvučnom ekstrakcijom organskim otapalom (USE). Analiza izoliranih hlapljivih spojeva uzoraka provedena je metodom plinska kromatografija - spektrometrija masa (GC-MS). Također je izvršena FTIR analiza gdje se dobila opća slika sastava meda od komorača. Mikroekstrakcijom vršnih para na krutoj fazi pomoću sivog vlakna izolirano je 26 hlapljivih spojeva, a pomoću plavog vlakna izolirano je 18 spojeva. Ultrazvučnom ekstrakcijom organskim otapalom izolirano je 13 spojeva. FTIR analizom je utvrđeno da ima razlike u krivulji meda od komorača i glukoznog sirupa.In this paper was, the chemical composition of fennel honey is analyzed. Volatile compounds were isolated by headspace solid phase microextraction (HS-SPME) with two different fibers (grey fiber and blue fiber) and organic solvent ultrasonic extraction (USE). The analysis of the isolated volatile compounds of the samples was performed by the gas chromatography-mass spectrometry (GC-MS) method. FTIR analysis was also performed, where a general picture of the composition of fennel honey was obtained. By microextraction of the peak pairs on the solid phase, 26 volatile compounds were isolated using the gray fiber and 18 compounds were isolated using the blue fiber. 13 compounds were isolated by ultrasonic extraction with an organic solvent. It was determined by spectroscopy (FTIR- ATR) that the curve of honey differ from the curve of glucose syrup