Add to ORKGIn molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications
Includes bibliographical references (pages 50-57)Cellulase is widely used as an enzyme for saccharif...
Advisors: Shengde Zhou.Committee members: Neil Blackstone; Jozef Bujarski; Ana Calvo; Stuart Hill; R...
Abstract Background Lignocellulosic ethanol could offer a sustainable source to meet the increasing ...
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene l...
209-211The transformation efficiency (TR)E of CaCl2-treated competent E. coli cells by plasmid DNA ...
This paper describes an efficient bacterial transformation system that was established for the prepa...
DNA manipulation routinely requires competent bacteria that can be made using one of numerous method...
Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion ...
International audienceTo finalize an effective and reproducible electroporation procedure to transfo...
This paper describes an efficient bacterial transformation system that was established for the prep...
Authors: Chenzhong Kuang ### Material and Reagents 1. SOC - 2% Tryptone - 0.5% Yeast Extract...
Bacterial cells can be engineered to express non-native genes, resulting in the production of, recom...
© 2020 Engineering microbes to utilize non-conventional substrates could create short and efficient ...
This project aims to develop products that simplify the laboratory methods used for the genetic imp...
Cheese whey is a by-product of the dairy industry and contains approximately 5% lactose, 0. 9% nitro...
Includes bibliographical references (pages 50-57)Cellulase is widely used as an enzyme for saccharif...
Advisors: Shengde Zhou.Committee members: Neil Blackstone; Jozef Bujarski; Ana Calvo; Stuart Hill; R...
Abstract Background Lignocellulosic ethanol could offer a sustainable source to meet the increasing ...
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene l...
209-211The transformation efficiency (TR)E of CaCl2-treated competent E. coli cells by plasmid DNA ...
This paper describes an efficient bacterial transformation system that was established for the prepa...
DNA manipulation routinely requires competent bacteria that can be made using one of numerous method...
Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion ...
International audienceTo finalize an effective and reproducible electroporation procedure to transfo...
This paper describes an efficient bacterial transformation system that was established for the prep...
Authors: Chenzhong Kuang ### Material and Reagents 1. SOC - 2% Tryptone - 0.5% Yeast Extract...
Bacterial cells can be engineered to express non-native genes, resulting in the production of, recom...
© 2020 Engineering microbes to utilize non-conventional substrates could create short and efficient ...
This project aims to develop products that simplify the laboratory methods used for the genetic imp...
Cheese whey is a by-product of the dairy industry and contains approximately 5% lactose, 0. 9% nitro...
Includes bibliographical references (pages 50-57)Cellulase is widely used as an enzyme for saccharif...
Advisors: Shengde Zhou.Committee members: Neil Blackstone; Jozef Bujarski; Ana Calvo; Stuart Hill; R...
Abstract Background Lignocellulosic ethanol could offer a sustainable source to meet the increasing ...