Protocol suggested for dna extraction of jabuticaba trees

O presente trabalho teve por objetivo adaptar o protocolo descrito por Soto (2001), testar diferentes fontes (tecido vegetal) e estabelecer condi\ue7\uf5es de coleta e pr\ue9-tratamento de amostras para extra\ue7\ue3o de DNA de jabuticabeira ( Plinia cauliflora (DC.) Berg, Myrtaceae). Utilizaram-se folhas em desenvolvimento, folhas completamente expandidas, c\ue2mbio do caule e pec\uedolo foliar. O protocolo testado pode ser utilizado para extra\ue7\ue3o de DNA de jabuticabeira, pois proporcionou isolamento de DNA de boa qualidade e possibilitou amplifica\ue7\ue3o por meio de PCR (\u201cRea\ue7\ue3o de Polimerase em Cadeia\u201d). Para evitar a oxida\ue7\ue3o de folhas \ue9 necess\ue1rio realizar a macera\ue7\ue3o com nitrog\ueanio l\uedquido.This study aimed to adapt the protocol described by Soto (2001), to test different sources (plant tissue) and to establish conditions for collection and pre-treatment of samples for extracting DNA from jabuticaba trees ( Plinia cauliflora (DC.) Berg, Myrtaceae). For this purpose, developing and expanded leaves, cambium of the stem and leaf stalk were used. The protocol tested can be used for DNA extraction from jabuticaba trees, because it provided DNA isolation of good quality and allowed the amplification of DNA through of PCR (Polymerase Chain Reaction). The use of liquid nitrogen for the maceration of leaves is necessary to prevent oxidation