\u418\u434\u435\u43d\u442\u438\u444\u438\u43a\u430\u446\u438\u44f \u41d\u430 \u429\u430\u43c\u43e\u432\u435, \u418\u437\u43e\u43b\u438\u440\u430\u43d\u438 \u412 \u422\u430\u439\u43b\u430\u43d\u434 \u418 \u41e\u442\u43d\u435\u441\u435\u43d\u438 \u41a\u44a\u43c \u420\u43e\u434\u43e\u432\u435\u442\u435 Kozakia \u418 Swaminathania

Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora (\u201ekhem\u201d in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans .\u418\u437\u441\u43b\u435\u434\u432\u430\u43d\u438 \u441\u430 \u442\u430\u43a\u441\u43e\u43d\u43e\u43c\u438\u447\u43d\u43e \u447\u435\u442\u438\u440\u438 \u449\u430\u43c\u430, \u438\u437\u43e\u43b\u438\u440\u430\u43d\u438 \u441\u44a\u43e\u442\u432\u435\u442\u43d\u43e \u43e\u442 \u43f\u43b\u43e\u434 \u43d\u430 \u441\u430\u43f\u43e\u434\u438\u43b\u430 \u432 \u427\u430\u43d\u442\u430\u431\u443\u440\u438, \u43e\u437\u43d\u430\u447\u435\u43d\u438 \u43a\u430\u442\u43e CT8-1 \u438 CT8-2, \u438 \u43e\u442 \u441\u435\u43c\u435\u43d\u430 \u43d\u430 \u438\u43a\u441\u43e\u440\u430 (\u201e\u43a\u435\u43c\u201d \u43d\u430 \u442\u430\u439\u432\u430\u43d\u441\u43a\u438) \u432 \u420\u430\u439\u43e\u43d\u433 \u438 \u43e\u437\u43d\u430\u447\u435\u43d\u438 \u43a\u430\u442\u43e SI15-1 and SI15-2. \u427\u435- \u442\u438\u440\u438\u442\u435 \u449\u430\u43c\u430 \u441\u430 \u43f\u43e\u434\u431\u440\u430\u43d\u438 \u43e\u442 \u43e\u431\u449\u43e 181 \u438\u437\u43e\u43b\u430\u442\u430 \u43d\u430 \u43e\u446\u435\u442\u43d\u43e\u43a\u438\u441\u435\u43b\u438 \u431\u430\u43a\u442\u435\u440\u438\u438. \u429\u430\u43c\u43e\u432\u435 CT8-1 \u438 CT8-2 \u441\u430 \u43d\u435\u43f\u43e\u434\u432\u438\u436\u43d\u438 \u438 \u43f\u440\u43e\u434\u443\u446\u438\u440\u430\u442 \u43b\u435\u432\u430\u43d\u43e\u43f\u43e\u434\u43e\u431\u435\u43d \u43c\u443\u43a\u43e- \u43f\u43e\u43b\u438\u437\u430\u445\u430\u440\u438\u434 \u43e\u442 \u437\u430\u445\u430\u440\u43e\u437\u430 \u438\u43b\u438 D-\u444\u440\u443\u43a\u442\u43e\u437\u430, \u43d\u43e \u43d\u435 \u43e\u442\u434\u435\u43b\u44f\u442 \u432\u43e\u434\u43d\u43e\u440\u430\u437\u442\u432\u43e\u440\u438\u43c \u43a\u430\u444\u44f\u432 \u43f\u438\u433\u43c\u435\u43d\u442 \u43e\u442 D-\u433\u43b\u44e\u43a\u43e\u437\u430 \u432\u44a\u440\u445\u443 \u441\u43a\u43e\u441\u435\u43d \u430\u433\u430\u440, \u441\u44a\u434\u44a\u440\u436\u430\u449 CaCO3. \u418\u437\u43e\u43b\u430- \u442\u438\u442\u435 \u43f\u440\u43e\u438\u437\u432\u435\u436\u434\u430\u442 \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 \u43e\u442 \u435\u442\u430\u43d\u43e\u43b \u438 \u43e\u43a\u438\u441\u43b\u44f\u432\u430\u442 \u430\u446\u435\u442\u430\u442 \u438 \u43b\u430\u43a\u442\u430\u442 \u434\u43e \u432\u44a\u433\u43b\u435\u440\u43e\u434\u435\u43d \u434\u432\u443\u43e\u43a\u438\u441 \u438 \u432\u43e\u434\u430, \u43d\u43e \u438\u43d\u442\u435\u43d\u437\u438\u432\u43d\u43e\u441\u442\u442\u430 \u43d\u430 \u43e\u43a\u438\u441\u43b\u435\u43d\u438\u435\u442\u43e \u435 \u441\u43b\u430\u431\u430. \u422\u435\u445\u43d\u438\u44f\u442 \u440\u430\u441\u442\u435\u436 \u43d\u435 \u441\u435 \u438\u43d\u445\u438\u431\u438\u440\u430 \u43e\u442 0.35 % \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 (v/v) \u43f\u440\u438 pH 3.5. \u418\u437\u43e\u43b\u430\u442\u438\u442\u435 \u43d\u435 \u440\u430\u441\u442\u430\u442 \u432\u44a\u440\u445\u443 30 % D-\u433\u43b\u44e\u43a\u43e\u437\u430 (w/v), \u430 \u443\u441\u432\u43e\u44f\u432\u430\u43d\u435 \u43d\u430 \u43c\u435\u442\u430\u43d\u43e\u43b \u43d\u435 \u441\u435 \u443\u441\u442\u430\u43d\u43e\u432\u44f\u432\u430. \u429\u430\u43c\u43e\u432\u435 SI15-1 \u438 SI15-2 \u438\u43c\u430\u442 \u43f\u435\u440\u438\u442\u440\u438\u445\u430\u43b\u43d\u43e \u440\u430\u437\u43f\u43e\u43b\u43e\u436\u435\u43d\u438 \u440\u435\u441\u43d\u438 \u438 \u440\u430\u441\u442\u430\u442 \u432 \u43f\u440\u438\u441\u44a\u441\u442\u432\u438\u435 \u43a\u430\u43a\u442\u43e \u43d\u430 0.35 % \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 (v/v) \u43f\u440\u438 pH 3.5, \u442\u430\u43a\u430 \u438 \u43d\u430 3 % NaCl (w/v) \u438\u43b\u438 1 % KNO3 (w/v). \u410\u446\u435\u442\u430\u442\u44a\u442 \u438 \u43b\u430\u43a\u442\u430\u442\u44a\u442 \u441\u435 \u43e\u43a\u438\u441\u43b\u44f\u432\u430\u442 \u434\u43e \u432\u44a\u433\u43b\u435\u440\u43e\u434\u435\u43d \u434\u432\u443\u43e\u43a\u438\u441 \u438 \u432\u43e\u434\u430, \u43d\u43e \u438\u43d\u442\u435\u43d\u437\u438\u432\u43d\u43e\u441\u442\u442\u430 \u435 \u441\u43b\u430\u431\u430. \u418\u437\u43e\u43b\u430\u442\u438\u442\u435 \u440\u430\u441\u442\u430\u442 \u432\u44a\u440\u445\u443 \u430\u433\u430\u440 \u441 \u43c\u430\u43d\u438\u442\u43e\u43b \u438\u43b\u438 \u433\u43b\u443\u442\u430\u43c\u430\u442, \u43a\u430\u43a\u442\u43e \u438 \u432\u44a\u440\u445\u443 30 % D-\u433\u43b\u44e\u43a\u43e\u437\u430 (w/v), \u43d\u43e \u43d\u435 \u443\u441\u432\u43e\u44f\u432\u430\u442 \u43c\u435\u442\u430\u43d\u43e\u43b. \u41f\u440\u43e\u432\u435\u434\u435\u43d\u438\u442\u435 \u441\u435\u43a\u432\u435\u43d- \u446\u438\u43e\u43d\u435\u43d \u430\u43d\u430\u43b\u438\u437 \u43d\u430 16S \u440\u420\u41d\u41a \u438 \u414\u41d\u41a-\u414\u41d\u41a \u445\u438\u431\u440\u438\u434\u438\u437\u430\u446\u438\u44f \u43f\u43e\u43a\u430\u437\u432\u430\u442, \u447\u435 \u449\u430\u43c\u43e\u432\u435 CT8-1 and CT8-2 \u431\u435\u437 \u441\u44a\u43c\u43d\u435\u43d\u438\u435 \u441\u435 \u438\u434\u435\u43d\u442\u438\u444\u438\u446\u438\u440\u430\u442 \u43a\u430\u442\u43e Kozakia baliensis , \u430 \u438\u437\u43e\u43b\u430\u442\u438 SI15-1 \u438 SI15-2 \u43a\u430\u442\u43e Swaminathania salitolerans