Emergence of colistin resistance in Klebsiella pneumoniae ST15 disseminating blaKPC-2 in a novel genetic platform
- Alvarez, Verónica Elizabeth
- Masso, Mariana Guillermina
- D'amico González, Gabriela Elena Noemí
- Gambino, Anahí Samanta
- Knecht, Camila Ayelén
- Prack Mc Cormick, Bárbara Patricia
- Leguina, Ana Carolina del Valle
- Piekar, María
- Poklepovich, Tomás
- Campos, Josefina
- Arduino, Sonia Marina
- Centron, Daniela
- Quiroga, María Paula
DOIAbstract
Objectives: Isolation of colistin- and carbapenem-resistant Klebsiella pneumoniae (CCR-Kp) is increasing in hospital settings worldwide, which is related to increased morbidity, mortality and healthcare costs. The aim of this work was to perform whole-genome sequencing (WGS), genomic and phylogenetic analysis, and conjugation assays of an extensively drug-resistant (XDR) CCR-Kp isolate from Argentina. Methods: WGS of strain KpS26 isolated from a bloodstream infection was performed using Illumina MiSeq-I, and de novo assembly was achieved using SPAdes v.3.11. A maximum likelihood tree was created using MEGA7 based on core genome single nucleotide polymorphisms from whole-genome alignment of K. pneumoniae isolates identified in silico as sequ...
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