The effect of selected herbicides on UGTs expression and activity of antioxidant enzymes in Arabidopsis thaliana

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Samanta Rysová Supervisor: doc. Ing. Petra Matoušková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: The effect of selected herbicides on UGTs expression and activity of antioxidant enzymes in Arabidopsis thaliana Herbicides are widely used to control weeds in agriculture. Although their use is essential to ensure crop production, their effect occurs to be reduced due to the development of herbicide resistence. Therefore it is important to clarify the mechanism of herbicide resistence, so that herbicides can be used effectively and new substances can be developed if necessary. At the same time, herbicides acts as an abiotic stress factor on the plant, thus affecting the antioxidant apparatus of plant cells. The aim of this work was to investigate the effect of selected herbicides - tribenuron-methyl (TBM), pinoxaden (PNX) and sulcotrione (SLC), on the expression of selected UGTs that could play a role in herbicide resistence. Secondary, we want to find out the effect of TBM on the activity of selected antioxidant enzymes - ascorbate peroxidase (APX), catalase (CAT) and superooxide dismutase (SOD). Arabidopsis thaliana was used as a model plant and the experiment was..