Glycerol is essential for growth in the 0% MM.

Growth curves of WT cells in the MM without glucose and with 3% glycerol (black) and that without both glucose and glycerol (red). Cells were precultured in the MM with 3% glucose and 3% glycerol. Each line represents an average of n = 2 samples. The data underlying this figure can be found in S2 Data. (PDF)