RAD-51 focus accumulation is delayed in aged unmated <i>rad-54</i> mutant germlines.

Violin and box plots depicting the number of RAD-51 foci in early pachytene germline nuclei of young (day 1 adult) and aged (day 4 adult) mated or unmated animals. Nuclei were quantified based on their positions in rows progressing through the germline on the distal proximal axis. Row 1 indicates the first row of nuclei in early pachytene. P values were calculated by pairwise Mann-Whitney U tests within each row of nuclei with Bonferroni correction for multiple comparisons. For simplicity, only significant (corrected p value ≤0.05) comparisons are displayed. Nuclei were scored from 6 germlines for each age and mating group (young, aged mated, aged unmated) derived from ≥2 experimental replicates. Each violin and boxplot represents an average of 29.5±3.3 nuclei (minimum 24 nuclei, maximum 37 nuclei, median 29 nuclei). Numerical data associated with this figure is presented in S2 Data. (TIF)