A) Stacked bar plot of the percent of nuclei in young (1 day post L4) and old (6 days post L4) unmated or sperm depleted fog-2 mutants with the given number of GFP::COSA-1 foci. See Fig 2A for an outline of the maintenance schemes used in each of these cohorts. The p value displayed was calculated by Chi Square test comparing the proportion of nuclei with >6, 6, or fog-2 mutant germlines. Scale bars represent 20μm. Germlines are oriented with the proximal end on the left and distal end on the right. Vertical low opacity white lines designate the end of pachytene. Numerical data associated with this figure is presented in S4 Data.</p
Bar plots representing the population brood viability of mated fog-2 mutant females. In this experim...
<p>Bar graph showing the extent of the region of DSB-2 positive nuclei in germ lines of indicated ge...
A) Schemes used to isolate young (1 day post-L4) and aged (4 days post L4) uev-2 mutant worms for ex...
Representative images of fog-2(q71) feminized mutant germlines from animals 1, 3, or 4 days post-L4....
A) Representative images of germlines from young and aged irradiated and unirradiated fog-2(q71) ger...
A) Schemes used to isolate young (1 day post-L4), aged (4 days post L4), and old (6 days post L4) fo...
A) Representative images of germlines stained with DSB-2. Solid lines indicate the “DSB-2 zone”, def...
Violin and box plots depicting the number of RAD-51 foci in early pachytene germline nuclei of young...
<p>(A–B) Histograms depict the quantification of RAD-51 foci in (A) control and (B) <i>lab-1(RNAi)</...
Region of wild type young and aged germlines which is stained with DSB-2. Each row represents a sing...
(A) Whole gonad immunostaining with DAPI (blue) and α-GFP (green) antibody marking GFP::MSH-5 in the...
<p>A. A dissected germline stained with DAPI (blue) and antibodies against SUN-1 pSer8 (red) and PCH...
A) Number of GFP::COSA-1 foci in mid-late pachytene in indicated mutants. Number of nuclei from a mi...
<p>Open bars denote fresh gametes (30 minutes after bundle breakup) and shaded bars denote aged game...
A) Schemes used to isolate young (1 day post-L4) and aged (4 days post L4) worms for experiments. Da...
Bar plots representing the population brood viability of mated fog-2 mutant females. In this experim...
<p>Bar graph showing the extent of the region of DSB-2 positive nuclei in germ lines of indicated ge...
A) Schemes used to isolate young (1 day post-L4) and aged (4 days post L4) uev-2 mutant worms for ex...
Representative images of fog-2(q71) feminized mutant germlines from animals 1, 3, or 4 days post-L4....
A) Representative images of germlines from young and aged irradiated and unirradiated fog-2(q71) ger...
A) Schemes used to isolate young (1 day post-L4), aged (4 days post L4), and old (6 days post L4) fo...
A) Representative images of germlines stained with DSB-2. Solid lines indicate the “DSB-2 zone”, def...
Violin and box plots depicting the number of RAD-51 foci in early pachytene germline nuclei of young...
<p>(A–B) Histograms depict the quantification of RAD-51 foci in (A) control and (B) <i>lab-1(RNAi)</...
Region of wild type young and aged germlines which is stained with DSB-2. Each row represents a sing...
(A) Whole gonad immunostaining with DAPI (blue) and α-GFP (green) antibody marking GFP::MSH-5 in the...
<p>A. A dissected germline stained with DAPI (blue) and antibodies against SUN-1 pSer8 (red) and PCH...
A) Number of GFP::COSA-1 foci in mid-late pachytene in indicated mutants. Number of nuclei from a mi...
<p>Open bars denote fresh gametes (30 minutes after bundle breakup) and shaded bars denote aged game...
A) Schemes used to isolate young (1 day post-L4) and aged (4 days post L4) worms for experiments. Da...
Bar plots representing the population brood viability of mated fog-2 mutant females. In this experim...
<p>Bar graph showing the extent of the region of DSB-2 positive nuclei in germ lines of indicated ge...
A) Schemes used to isolate young (1 day post-L4) and aged (4 days post L4) uev-2 mutant worms for ex...