Change in global expression patterns correlates with the change in hybridness.

(A) Depiction of JSD as a measure of distance between two frequency distributions. (B) JSD values were obtained for the single edge perturbation networks of the 4N 7E EMP network. The edge perturbation indices are given in S1 Table. (C) Bar plot showing the frequency of Epithelial, Mesenchymal, and Hybrid phenotypes for WT 4N 7E EMP network, perturbation with least JSD (Snail-miR200-2-1), and perturbation with highest JSD (ZEB-GRHL2-2-1). (D) Representative scatter plots between JSD and Hybridness for (i) 4N 7E, (ii) 10N 22E, and (iii) 22N 82E. Each point corresponds to one perturbation. Spearman correlation coefficient is reported. (E) Depiction of J metric calculation with the example of 4N 7E network correlation matrix. (F) Representative scatter plots between J metric and Hybridness for (i) 4N 7E and (ii) 22N 82E. Spearman correlation coefficient is reported. (G) Spearman correlation between hybridness and JSD (top row) and J metric (bottom row). “X” represents p-value > 0.05. *: p-value < 0.05.