2-AG signaling inhibits nuclear translocation of DAF-16 induced by cholesterol depletion.

(A) DAF-16::GFP nuclear translocation group classification. Translocation categories were placed into 3 classes as shown in the picture. Animals which exhibited DAF-16::GFP nuclear accumulation between 0–10% of total DAF-16::GFP were classified as Low Translocation (LowT), 30–50% as Middle Translocation (MiddleT) and 70–90%, High Translocation (HighT). All animals shown are in a L2 larva stage. (B) Nuclear translocation of DAF-16::GFP in worms grown under normal cholesterol concentration (CHOL) and after cholesterol depletion for two generations in the absence (solvent) or presence of 2-AG. 69 individual worms were analyzed for the solvent condition, 66 for 2-AG and 64 for CHOL.