Fig 6 -

A. 1D free energy profiles for RNA dissociation from the RRMs (left), RBD (middle) and SUMO2/RBD complex (right), using R, the distance between RNA and the RNA binding pocket in RRMs, as the order parameter. The dashed black lines indicate a free energy difference of 8 kcal/mol from the RNA-bound state. B. 2D free energy landscapes for the RNA dissociation process from RRMs (left), RBD (middle) and SUMO2/RBD complex (right), using the principal component PC0 and R as two order parameters. C. Representative structures sampled from along the RNA dissociation pathway I from RRMs, as the dashed black arrow shown in Fig 6B. D. Representative structures sampled from along the RNA dissociation pathway II from SUMO2/RBD complex, as shown in Fig 6B. The main chain of the target RNA is colored in white and orange.