Evaluation of changes in rat fatigability and biochemical parameters after oral and intra-peritoneal administration of adenosine tri phosphate: an experimental study

Background: Adenosine tri phosphate (ATP) is an important intracellular energy source and has many extracellular functions meadiacating through purine receptors. Currently ATP is available in the market as oral dietary supplement. However there are inconclusive studies regarding its efficacy through oral route. Hence this study was carried out to evaluate efficacy of oral and intraperitoneal administration of ATP in experimental rats by comparing swim exhaust time and associated biochemical changes.Methods: 18 Swiss albino rats of either gender were grouped randomly into three groups, consisting of group -1 control group which receive distilled water (5ml/kg body weight) whereas group 2 and 3 received oral and intraperitoneal ATP (60mg/kg body weight) for 8 days. On 8th day all rats all rats were evaluated for extent of physical fatigue by using exhaustive swimming test time required to attain immobility status is noted. Under aseptic precautions blood samples were drawn from rat tail vein and biochemical parameters like uric acid, triglycerides, total cholesterol, random blood sugar and c-reactive protein levels were measured.Results: There was a significant (p<0.05) increase in serum uric acid, blood sugar and urea in Group 2and3 compared control group 1. There was no statistically significant increase in physical strength in group 2 and 3 as compared to group 1.Conclusions: Oral and intraperitoneal administration of ATP may lead to hyperglycaemia, hyperuricemia and dyslipidaemia without significant increase in muscle strength