Biodegradation of Used Lubricating Oil Containing Hydrocarbon using Pseudomonas Aeruginosa and Rhodococcus Erythopolis

Engine oil is complex mixture of hydrocarbons and organic compounds used to lubricate parts car engine so the engine workssmoothly. After the usage period the oil as a lubricant ends, then the oil will be used more metals and polycyclic aromatichydrocarbons (PAH). One friendly way environment is by bioremediation, namely biodegradation of pollutant compounds become simpler products and harmless. So far, research on contamination of hydrocarbon compounds especially used oil in fishing ports is still rarely done, especially with the addition of exogenous bacteria. This study aims to analyze the ability of the bacterium Pseudomonas aeuriginosa, Rhodococcus erythropolis and the combination of both in the bioremediation process of used oil hydrocarbons and determine the best bacterial formulations to degrade hydrocarbons from used oil waste. The method used in this research is the experimental method. Significant reduction results occurred in the treatment of combined Pseudomonas aeruginosa and Rhodococcus erythopolis with concentrations of used oil 30 ppm by 70% with the value of used oil concentration of 9 ppm, then in the treatment of Rhodococcus erythopolis with 45 ppm concentration of used oil there was a percentage the lowest decrease of 22% with the value of used oil final concentration of 35 ppm. If referring to the Regulation of the Minister of Environment No. 19 of 2010 that bioremediation of used oil waste using a combination of Pseudomonas aeruginosa and Rhodococcus erythopolis can produce final value pollutants that are still allowed for the harbor's aquatic environment