Not Available

Not AvailableAn experiment was conducted to monitor temporal changes in the gene expression of a specific molecular marker, HIF-1a in response to a 15-day exposure to chronic hypoxia in a bottom dwelling carp, Cirrhinus mrigala. The expression level of HIF-1a mRNA was increased in gills of C. mrigala in response to 0.5 mg/L, dissolved oxygen (DO) for 15 days with the time of exposure, whereas it remained unchanged in fishes exposed to normoxia (DO, 6.5 mg/L). Based on the first experiment it is found that HIF is expressed at moderate level 72 hours, and it peaks at 7 and 15 days, indicating that 72 h is a tolerable time limit for survival under hypoxia for the particular species. Thus, a second experiment was conducted to evaluate the effect of hypoxia (0.5 mg/L) for 72 h and assessed different parameters such as metabolic enzymes, antioxidant enzymes and haematological parameters. The haematological parameters, such as RBC count, Hb and Hct values increased significantly (P