Keberadaan bakteri multiresisten Escherichia coli penghasil ESBL/AmpC, Klebsiella oxytoca dan Kluyvera spp. diisolasi dari lingkungan ayam rumah potong hewan di Pondok Rumput, Bogor, Indonesia: Keberadaan bakteri multiresisten Escherichia coli penghasil ESBL/AmpC, Klebsiella oxytoca dan Kluyvera spp. diisolasi dari lingkungan ayam rumah potong hewan di Pondok Rumput, Bogor, Indonesia

Dalam beberapa tahun terakhir, bakteri resisten multidrug meningkat secara signifikan dan mencemari karkas ayam melalui rantai produksi. Penelitian ini mengidentifikasi keberadaan Enterobactericeae penghasil ESBL/AmpC yang dikumpulkan dari lingkungan pemotongan ayam. Sampel diambil sebelum proses penyembelihan dari beberapa titik lokasi yang telah ditentukan. Dari total 84 sampel yang diuji, 30 isolat positif Enterobactericeae terdiri dari 22 isolat E. coli, 6 isolat Klebsiella oxytoca, dan 1 isolat Kluyvera spp. Uji konfirmasi fenotipik ESBL/AmpC dan uji resisten antibiotik terhadap 18 jenis antibiotik dilakukan dengan menggunakan kit komersial MASTDISCS D68C dan uji kepekaan difusi cakram Kirby-Bauer dengan interpretasi CLSI (2014). Semua isolat menunjukkan resistensi terhadap penisilin, ampisilin, amoksisilin, sefotaksim, sefpodoksim, seftazidim dan beberapa isolat menunjukkan resistensi terhadap streptomisin, gentamisin, trimetoprim-sulfametoksasol, tetrasiklin, kanamisin, doksisiklin, colistin sulfat, sefalotin dan polimiksin, neomisin. keberadaan Enterobactericeae penghasil ESBL/AmpC yang ditemukan pada wadah karkas, wadah jeroan, lantai karkas, area penanganan jeroan, pisau dan mesin penarik bulu. Adanya bakteri resisten di lingkungan pemotongan ayam dapat menyebabkan terjadinya resistensi antibiotik dan transmisi gen resistensi terhadap bakteri lain yang dapat menyebar ke karkas yang berpotensi merugikan manusia.In the last few years, multidrug resistant bacteria increased significantly and contaminated chicken carcass through production chain. This study identified the presence of ESBL/AmpC-producing Enterobactericeae collected from the environment of chicken slaughterhouses. Samples were taken before slaughtering process from several points of determined locations. From a total of 84 samples tested, 30 isolates were positive of Enterobactericeae comprising of 22 isolates of E. coli, 6 isolates of Klebsiella oxytoca, and 1 isolate of Kluyvera spp. Confirmation test of ESBL/AmpC phenotypic and antibiotic resistant test to 18 kinds of antibiotics were conducted using MASTDISCS D68C commercial kit and Kirby-Bauer disc diffusion susceptibility test with CLSI (2014) interpretation. All of isolates showed resistance to penicillin, ampicillin, amoxicillin, cefotaxime, cefpodoxime, ceftazidime and some isolates show resistance to streptomycin, gentamicin, trimethoprim-sulphametoxasol, tetracycline, kanamycin, doxycyclin, colistin sulphate, cephalothin, neomycin and polymyxin  B. This study demonstrated the existence of ESBL/AmpC-producing  Enterobactericeae that were found on the carcasses container, offal container, floors of carcasses, offal handling area, knives and feathers puller machine. The existence of resistant bacteria in the environment of chicken slaughterhouse can lead to occurrence of antibiotic resistant and transmission of resistance genes to other bacteria that could spread to the carcass that was potentially harmful to humans