Manufacturing a modified carbon paste electrode with catalase enzyme-Au nanoparticles for electrochemical sensing of hydrogen peroxide and their electrocatalytic properties

To find hydrogen peroxide, different techniques applied, recently. In this practice we decided to produce a modified carbon paste electrode by catalase enzyme-Au nanoparticles for electrochemical sensing hydrogen peroxide and their electroactivity characteristics. Cyclic voltammetry were done electrochemical researches. A three-electrode method including a modified carbon paste electrode with catalase enzyme-Au nanoparticles as the operant electrode, a platinum string electrode as a counter electrode, and saturated calomel electrode as a reference electrode, was applied. Cyclic voltammetric assays were done with different scan speed area from of 50 mV s−1 to 500 mV s−1. Transmission electron microscopy was checked external morphological characteristics of Au nanoparticles. H2O2 in 100 μM to 450 μM area could find out by designed biosensor. By perform assays in two weeks regular interval, the resistance of modified carbon paste electrode with catalase enzyme-Au nanoparticles biosensor has been determine and it has been discovered that after 14 days, modified carbon paste electrode with catalase enzyme-Au nanoparticles keeps its 97% activity. Keywords: electrochemical sensing, hydrogen peroxide, catalase enzyme, Au nanoparticle