CRISPR-based systems have fundamentally transformed our ability to study and manipulate stem cells. We explored the possibility of using catalytically dead Cas9 (dCas9) from S. pyogenes as a platform for targeted epigenetic editing in stem cells to enhance the expression of the eomesodermin gene (EOMES) during differentiation. We observed, however, that the dCas9 protein itself exerts a potential non-specific effect in hiPSCs, affecting the cell's phenotype and gene expression patterns during subsequent directed differentiation. We show that this effect is specific to the condition when cells are cultured in medium that does not actively maintain the pluripotency network, and that the sgRNA-free apo-dCas9 protein itself influences endogenou...
SummaryCRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression i...
With their intrinsic capacity to self-renew and their potential to differentiate to specialized tiss...
Pluripotent cells possss the ability to self-renew or differentiate into somatic cell types based on...
The identification of the trans-acting factors and cis-regulatory modules that are involved in human...
Epigenetic editing is a promising approach to modulate the local chromatin environment of target gen...
Human pluripotent stem cells (hPSCs) and mesenchymal stromal/stem cells (hMSCs) are clinically relev...
The directed differentiation of stem cells has a wide range of applications in fields such as regene...
Along with functional advances in the use of CRISPR/Cas9 for genome editing, endonuclease-deficient ...
CRISPR-Cas9 effector systems have wide applications for the stem cell and regenerative medicine fiel...
Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors...
Human-induced pluripotent stem cells (hiPSCs) and CRISPR/Cas9 gene editing system represent two inst...
Objective:CRISPR/Cas9 technology has revolutionized gene editing and fast tracked our capacity to ma...
CRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression in human...
We report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived huma...
Targeted transcriptional regulation is a powerful tool to study genetic mediators of cellular behavi...
SummaryCRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression i...
With their intrinsic capacity to self-renew and their potential to differentiate to specialized tiss...
Pluripotent cells possss the ability to self-renew or differentiate into somatic cell types based on...
The identification of the trans-acting factors and cis-regulatory modules that are involved in human...
Epigenetic editing is a promising approach to modulate the local chromatin environment of target gen...
Human pluripotent stem cells (hPSCs) and mesenchymal stromal/stem cells (hMSCs) are clinically relev...
The directed differentiation of stem cells has a wide range of applications in fields such as regene...
Along with functional advances in the use of CRISPR/Cas9 for genome editing, endonuclease-deficient ...
CRISPR-Cas9 effector systems have wide applications for the stem cell and regenerative medicine fiel...
Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors...
Human-induced pluripotent stem cells (hiPSCs) and CRISPR/Cas9 gene editing system represent two inst...
Objective:CRISPR/Cas9 technology has revolutionized gene editing and fast tracked our capacity to ma...
CRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression in human...
We report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived huma...
Targeted transcriptional regulation is a powerful tool to study genetic mediators of cellular behavi...
SummaryCRISPR/Cas9 protein fused to transactivation domains can be used to control gene expression i...
With their intrinsic capacity to self-renew and their potential to differentiate to specialized tiss...
Pluripotent cells possss the ability to self-renew or differentiate into somatic cell types based on...