We present the first estimate of the Ly α luminosity function using blind spectroscopy from the Multi Unit Spectroscopic Explorer, MUSE, in the Hubble Deep Field-South. Using automatic source-detection software, we assemble a homogeneously detected sample of 59 Ly α emitters covering a flux range of −18.0 < log10 (F) < −16.3 (erg s−1 cm−2), corresponding to luminosities of 41.4 < log10 (L) < 42.8 (erg s−1). As recent studies have shown, Ly α fluxes can be underestimated by a factor of 2 or more via traditional methods, and so we undertake a careful assessment of each object's Ly α flux using a curve-of-growth analysis to account for extended emission. We describe our self-consistent method for determining the completeness of the sample, and...