Characterization of Coliforms from Water of Ranchan River, Serian Sarawak

Coliform bacteria are Gram-negative belongs to the genera such as Citrobacter, Enterobacter, Klebsiella and Escherichia. Coliform bacteria are capable to cause pneumonia, respiratory diseases and urinary tract infections. As water provides as a potential transmission channel for pathogenic coliform bacteria, the rising cases of waterborne disease have raised concerns over the years. This research was carried out to isolate, identify, and characterize coliforms bacteria from water samples of Ranchan river, Sarawak. A total of 15 isolates were cultured, identified by 16S rRNA sequencing and characterized against 8 antibiotics. From the results, most bacteria (81.82%) present are resistant to Ampicillin (AMP,10). Result of the identification of bacteria by 16S rRNA sequencing revealed bacteria from the Enterobacteriaceae family, Pseudomonas aeruginosa, and Aeromonas. The antibiotic susceptibility test showed 100% of isolates were susceptible to Norfloxacin (NOR,10)