The protocol describes the procedure of antigen-specific T cell generation and TCR identification for the use in adoptive T cell therapy. We describe two paths of generating antigen-specific T cells, first, T cell stimulation with autologous dendritic cells pulsed with antigen peptide, second, in vivo T cell stimulation with peptide or DNA by gene gun application in a suitable mouse model followed by in vitro enrichment of peptide-reactive T cells. Peptide-stimulated T cells are sorted by fluorescence-activated cell sorting for CD107α or IFNγ expression and subsequently isolated RNA is used in a 5' rapid amplification of cDNA ends (RACE )-PCR specific for TCR for TCR chain identification. After retroviral cloning, it is re-expressed on huma...
Adoptive transfer of T cells expressing transgenic T-cell receptors (TCRs) with antitumor function i...
Adoptive transfer of T cells expressing transgenic T cell receptors (TCR) with anti-tumor function i...
Importance of the field: Adoptive therapy with T cell receptor- (TCR-) redirected T cells has shown ...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
T-cell receptor (TCR) gene therapy aims to induce immune reactivity against tumors by introducing ge...
The antigen specificity of T lymphocytes is dictated solely by the T cell receptor (TCR) alpha and b...
The antigen specificity of T lymphocytes is dictated solely by the T cell receptor (TCR) alpha and b...
A recent phase 1 trial has demonstrated that the generation of tumor-reactive T lymphocytes by trans...
T cell responses against tumor-antigens are frequently observed for some human malignancies, in part...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Designer T cells expressing transgenic T cell receptors (TCR) with anti-tumor specificity offer new ...
Adoptive transfer of T cells expressing transgenic T-cell receptors (TCRs) with antitumor function i...
Adoptive transfer of T cells expressing transgenic T cell receptors (TCR) with anti-tumor function i...
Importance of the field: Adoptive therapy with T cell receptor- (TCR-) redirected T cells has shown ...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haema...
T-cell receptor (TCR) gene therapy aims to induce immune reactivity against tumors by introducing ge...
The antigen specificity of T lymphocytes is dictated solely by the T cell receptor (TCR) alpha and b...
The antigen specificity of T lymphocytes is dictated solely by the T cell receptor (TCR) alpha and b...
A recent phase 1 trial has demonstrated that the generation of tumor-reactive T lymphocytes by trans...
T cell responses against tumor-antigens are frequently observed for some human malignancies, in part...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persis...
Designer T cells expressing transgenic T cell receptors (TCR) with anti-tumor specificity offer new ...
Adoptive transfer of T cells expressing transgenic T-cell receptors (TCRs) with antitumor function i...
Adoptive transfer of T cells expressing transgenic T cell receptors (TCR) with anti-tumor function i...
Importance of the field: Adoptive therapy with T cell receptor- (TCR-) redirected T cells has shown ...