Phenylalanine aminomutase from Taxus chinensis (TchPAM) is employed in the biosynthesis of the widely used antitumor drug paclitaxel. TchPAM has received substantial attention due to its strict enantioselectivity towards (R)-β-phenylalanine, in contrast to the bacterial enzymes classified as EC 5.4.3.11 which are (S)-selective for this substrate. However, the understanding of the isomerization mechanism of the reorientation and rearrangement reactions in TchPAM might support and promote further research on expanding the scope of the substrate and thus the establishment of large-scale production of potential synthesis for drug development. Upon conservation analysis, computational simulation, and mutagenesis experiments, we report a mutant f...
An aminomutase, naturally catalyzing the interconversion of (S)--phenylalanine and (R)--phenylalanin...
Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regiosele...
An approach is described for the synthesis of aromatic α- and β-amino acids that uses phenylalanine ...
Phenylalanine aminomutase from Taxus chinensis (TchPAM) is employed in the biosynthesis of the widel...
The phenylalanine aminomutase (PAM) from Taxus chinensis catalyses the conversion of α-phenylalanine...
By replacing a single active-site residue Cys107 with Ser in phenylalanine aminomutase (PAM), the en...
Lignin is an underutilized sustainable source of aromatic compounds. To valorize the low-value ligni...
Phenylalanine-2,3-aminomutase (PAM) from Taxus chinensis, a 4-methylidene-imidazole-5-one (MIO)-depe...
Phenylalanine-2,3-aminomutase (PAM) from <i>Taxus chinensis</i>, a 4-methylidene-imidazole-5-one (MI...
β-Amino acids are biologically active compounds of interest in medicinal chemistry. A class I lyase-...
An aminomutase, naturally catalyzing the interconversion of (S)--phenylalanine and (R)--phenylalanin...
Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regiosele...
An approach is described for the synthesis of aromatic α- and β-amino acids that uses phenylalanine ...
Phenylalanine aminomutase from Taxus chinensis (TchPAM) is employed in the biosynthesis of the widel...
The phenylalanine aminomutase (PAM) from Taxus chinensis catalyses the conversion of α-phenylalanine...
By replacing a single active-site residue Cys107 with Ser in phenylalanine aminomutase (PAM), the en...
Lignin is an underutilized sustainable source of aromatic compounds. To valorize the low-value ligni...
Phenylalanine-2,3-aminomutase (PAM) from Taxus chinensis, a 4-methylidene-imidazole-5-one (MIO)-depe...
Phenylalanine-2,3-aminomutase (PAM) from <i>Taxus chinensis</i>, a 4-methylidene-imidazole-5-one (MI...
β-Amino acids are biologically active compounds of interest in medicinal chemistry. A class I lyase-...
An aminomutase, naturally catalyzing the interconversion of (S)--phenylalanine and (R)--phenylalanin...
Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regiosele...
An approach is described for the synthesis of aromatic α- and β-amino acids that uses phenylalanine ...