Sviluppo di un corredo diagnostico commerciale per la certificazione dello stato fitosanitario delle produzioni vivaistiche di carciofo

Development of a commercial diagnostic kit for the certification of the phytosanitary status in nursery production of globe artichoke. Artichoke Italian Latent virus (AILV), Artichoke latent virus (ArLV), Artichoke mottled crinkle virus (AMCV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV), Pelargonium zonate spot virus (PZSV), Tomato infectious chlorosis virus (TICV), Tobacco mosaic virus (TMV), Tomato spotted wilt virus (TSWV) and Turnip mosaic virus (TuMV) are damaging to artichoke, so new artichoke fields should be planted with virus-free certified germplasm. We have developed a protocol for the simultaneous detection of these artichoke viruses by nonisotopic dot blot hybridisation with DNA probes. The probe mix detected all viruses with identical specificity to that obtained using individual probes. The kit is proposed for the routine assessment of phytosanitary status of certified nursery production of globe artichoke