O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B

Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-L-GulpNAc3OAcAN-(1 →3)-β-D-QuipNAc-(1→