The effect of quercetin on the developmental consequences of methamphetamine in fruit fly

Metamfetanim (METH) je psihostimulans koji dovodi do razvoja ovisnosti. Naši preliminarni rezultati su pokazali da METH dovodi do promjena u broju potomaka mužjaka vinske mušice. Razvojni ciklus je povezan s metabolizmom, a time i sa razvojem reaktivnih oksidacijskih vrsta. METH povećava stvaranje reaktivnih kisikovih vrsta (ROS) i ometa njihovo uklanjanje, uzrokujući oksidativni stres. Kvercetin (QUE) je molekula s poznatim antioksidativnim svojstvima koja bi potencijalno mogla djelovati na promjene u razvojnom ciklusu uzrokovane METH-om. Cilj eksperimentalnog rada je bio ispitati kako promjene u oksidacijskom statusu (QUE) utječu na broj potomaka mužjaka izloženih vMETH-a. METH je administriran grupi mužjaka pomoću FlyBong metode, nakon čega je izdvojeno 20% mužjaka s najvećim lokomotornim odgovorom na METH. Mužjaci su pareni sa netretiranim ženkama na hrani s različitim koncentracijama QUE (0.6 mM i 3.2 mM). Praćen je utjecaj QUE na broj potomaka tijekom tri generacije. Rezultati su pokazali statistički značajno povećanje broja embrija prve generacije u skupini koja je bila izložena METH-u kao i trend povećanog broja čahura i mušica. Utjecaji METH-a se nije prenio na naredne generacije, dok je utjecaj QUE-a bio vidljiv u drugoj, ali ne i trećoj generaciji. Rezultati upućuju na potencijalan transgeneracijski utjecaj QUE na redoks ravnotežu. Kako bi se ovi rezultati statstički potvrdili, eksperiment je potrebno ponoviti s povećanim brojem replika i poboljšanom formulacijom hrane.Methamphetamine (METH) is a psychostimulant that leads to the development of addiction. Our preliminary results showed that METH leads to changes in the number of male fruit fly offspring. The development cycle is connected with metabolism, and thus with the development of reactive oxidation species. METH increases the generation of reactive oxygen species (ROS) and interferes with their removal, causing oxidative stress. Quercetin (QUE) is a molecule with known antioxidant properties that could potentially act on METH-induced changes in the developmental cycle. The aim of the experimental work was to examine how changes in oxidation status (QUE) affect the number of offspring of males exposed to vMETH. METH was administered to a group of males using the FlyBong method, after which 20% of males with the highest locomotor response to METH were selected. Males were mated with untreated females on food with different concentrations of QUE (0.6 mM and 3.2 mM). The influence of QUE on the number of offspring was monitored over three generations. The results showed a statistically significant increase in the number of embryos of the first generation in the group that was exposed to METH, as well as a trend of an increased number of cocoons and flies. The effects of METH were not transmitted to subsequent generations, while the influence of QUE was visible in the second but not the third generation. The results suggest a potential transgenerational influence of QUE on redox balance. In order to statistically confirm these results, the experiment needs to be repeated with an increased number of replicates and an improved food formulation