Effect of nutrient medium on the specialized metabolites of two grepevines (Vitis vinifera L.) cultivars in vitro

Cilj ovoga rada bio je uzgojiti dvije sorte vinove loze 'Portan' i 'Graševinu' u uvjetima in vitro na dvije različite hranjive podloge, uobičajenoj podlozi koja sadrži Murashige i Skoog medij te podlozi koja osim navedenog medija sadrži i usporivač rasta, alar B9. Biljke uzgojene u uvjetima usporenoga rasta bile su manje u vidu kraćih stabljika i manjih dimenzija listova u odnosu na biljke uzgojene na uobičajenoj podlozi. Istražen je utjecaj različitih podloga na sastav i sadržaj pojedinačnih polifenolnih te hlapljivih organskih spojeva u listovima navedenih sorti. Veće vrijednosti masenih udjela gotovo svih analiziranih polifenolnih spojeva dobivene su u uzorcima listova biljaka uzgojenih na uobičajenoj podlozi, dok su gotovo svi pojedinačni hlapljivi spojevi bez obzira u koju kemijsku skupinu pripadaju (terpenoide, kiseline, aldehide, ketone i alkohole) imali veće apsolutne površine pikova u uvjetima usporenog rasta. Dobiveni rezultati potvrđuju da uporaba hranjive podloge za usporeni rast nema negativan utjecaj na biljku čime je utvrđena prikladnost njene uporabe za potrebe čuvanja genetskog materijala vinove loze u uvjetima in vitro, a čime se smanjuje učestalost subkultivacije biljaka.The aim of this work was to grow two grape varieties 'Portan' and 'Graševina' under in vitro conditions on two different nutrient media, the usual medium containing Murashige and Skoog medium and the medium containing, in addition to the mentioned medium, a growth retarder, alar B9. Plants grown under conditions of slow growth were smaller in terms of shorter stems and smaller leaf sizes compared to plants grown on the usual substrate. The influence of different nutrient media on the composition and content of individual polyphenolic and volatile organic compounds in the leaves of the mentioned varieties was tested. Higher values of content of almost all analyzed polyphenolic compounds were obtained in leaf samples of plants grown on the usual substrate, while almost all individual volatile compounds, regardless of which chemical group they belong to (terpenoids, acids, aldehydes, ketones, and alcohols), had greater responses in the slow growth conditions. The obtained results confirm that the use of nutrient medium for slow growth does not have a negative effect on the plant, which established the suitability of its use for the purposes of preserving the genetic material of the grapevine in vitro, which reduces the frequency of subcultivation of plants