Identifikasi Kerusakan Sel pada Hepar dan Ren Tikus Putih (Rattus norvegicus, L.) Jantan Setelah diberi Pakan Mie Berboraks selama 30 Hari Berturut-Turut

Penelitian deskriptif ini bertujuan mengidentifikasi kerusakan sel pada hepar dan ren tikus putih ( Rattus norvegicus L ) jantan yang diberi pakan mie berboraks selama 30 hari berturut-turut. Sampel yang digunakan dalam penelitian ini berjumlah 25 ekor tikus putih jantan yang dibagi menjadi 5 kelompok perlakuan yaitu P0 (kontrol, diberi 100% pelet), P1 (diberi 25% mie berboraks dan 75% pelet), P2 (diberi 50% mie berboraks dan 50% pelet), P3 (diberi 75% mie berboraks dan 25% pelet) dan P4 (diberi 100% mie berboraks). Setelah perlakuan selama 30 hari, tikus putih dibedah untuk diambil organ hepar dan ren-nya, kemudian organ diproses secara mikrotehnik dengan pewarnaan HE, dan diamati kerusakan selulernya dibawah mikroskop dengan perbesaran 10x hingga 40x. Hasil pengamatan menunjukkan bahwa ada kerusakan seluler baik pada hepar maupun ren, dengan jenis kerusakan didominasi bengkak keruh pada P1 dan P2, terjadi degenerasi pelemakan pada P2 dan P3, dan kerusakan permanen berupa nekrosis pada P4. Dari hasil penelitian dapat disimpulkan bahwa pemberian mie yang mengandung boraks selama 30 hari berturut-turut mengakibatkan kerusakan pada sel hepar dan renl tikus putih ( Rattus norvegicus, L ) jantan berupa bengkak keruh (cloudy swelling), degenerasi pelemakan dan nekrosis.This descriptive research is aimed to identify cell damaged on liver and kidney of Male White Rat ( Rattus norvegicus L ) after given of noodle contained borax in 30 consecutive days. 25 of male white rats were used as sample which were grouped into 5 treatment as followed P0 (control, given 100% pellet), P1 (given 25% noodle contained borax and 75% pellet), P2 (given 50% noodle contained borax and 50% pellet), P3 (given 75% noodle contained borax and 25% pellet) and P4 (given 100% noodle contained borax). After being treated with noodle contained borax in 30 consecutive days, all rats were dissected to take out the liver and kidney, then all organs were micro technically processed and stained with HE. Furthermore, all preparation were observed carefully under the microscope to see if some damaged cells occurred. The result shows that there were some damaged cells either in liver cells or kidney cells in the form of cloudy swelling (P1 and P2), fatty acid degeneration (P2 and P3), and necrotic in P4. So, it can be concluded that consuming noodle contained borax in 30 consecutive days for male white rat ( Rattus norvegicus, L ) will cause cloudy swelling, fatty acid degeneration and necrotic in liver and kidney cells