Prevalence of Tetracycline Resistance Genes in Staphylococcus Aureus Isolated From Different Clinical Sources in Diyala, Iraq

Antibiotic resistance showed by many bacterial species such as Staphylococcus aureus is a great global problem. This paper is included the prevalence percentage of some tetracycline's resistance genes in isolates of S.aureus collected from different sources. Seventy five different samples were collected from different sources, including wound, burn, nasal, blood, and urine samples from several hospitals of Baquba city and the samples cultured on two culture agar Blood Agar and Mannitol Salt Agar for studying of colony properties. Gram stain and some biochemical tests such as (coagulase, oxidase, catalase) were done for this purpose.  The molecular method was dependent on PCR in the detection of specific genes, 16SrRNA, to diagnose the staphylococcus genus. The results of 16SrRNA PCR detection revealed that fifteen isolates were identified as S. aureus. All the isolates were subjected to polymerase chain reaction to amplify four common tetracycline resistance determinants, tetK, tet38 ,tetL, tetM,. A number of 15 isolates (100%) were found to be positive for tetK genes  , Out of 15 resistant S.aureus isolates, 15 (100%) were found to produce tet38 genes,while  5(33.3%) isolates have tetL genes , 8(53.3%)  carried tetM genes