Screening for Suitable Parameters of Hydrolysis Rection of Red Meat of Striped Tuna (Sarda Orientalis) with HCL as A Catalyst to Obtain Protein Hydrolysate

The purpose of this research is to hydrolyze red meat of striped tuna (Sarda orientalis) with HCl as a catalyst to obtain protein hydrolysate which will overcome the shortcoming of previous methods, increase economic value of red meat of striped tuna, and reduce solid waste of tuna processing industry. Red meat of striped tuna is rich in protein and a perfect material for hydrolysis to obtain protein hydrolysate with 22.79 ± 0.69% of protein content. Suitable conditions of hydrolysis reaction are determined as follows: HCl concentration is 0.1 M, ratio of HCl volume to substrate is 34:01 (v:w), reaction time is 10 min, and reaction temperature is 30°C. With such suitable conditions of hydrolysis reaction, protein recovery yield reaches the maximal value of 65.26 ± 2.85%. A minor amount of amino acid is found in crude products. This research has provided some important information about applying HCl as a catalyst to hydrolyze by-product aquatic to obtain protein hydrolysate