Biosynthesis of titanium dioxide nanoparticles using Hypericum perforatum and Origanum vulgare extracts and their main components, hypericin and carvacrol as promising antibacterial agents

OBJECTIVE: To evaluate the anti-bacterial activities of titanium dioxide (TiO2) nanoparticles of Origanum (O.) vulgare and Hypericum (H.) perforatum extracts, carvacrol and hypericin against Staphylococcus (S.) aureus. METHODS: In this study, TiO2 nanoparticles of O. vulgare and H. perforatum extracts, carvacrol and hypericin, were prepared and their antibacterial effects were evaluated against Staphylococcus (S.) aureus. In this study, scanning electron microscope, fourier transform infrared spectrometer, atomic force microscopy, dynamic light scattering and zeta potential were used to investigate the structure of synthesized drugs. RESULTS: Anti-bacterial activity of synthesized NPs was tested by minimum inhibitory concentration (MIC), minimum bactericidal concentration and disc diffusion method. MICs of TiO2-NPs synthesized using O. vulgare, H. perforatum, carvacrol and hypericin and TiO2 were obtained 250, 62.5, 250, and 250, and 500 mu g/mL, respectively. The MBCs for all of these were obtained 1000 mu g/mL. CONCLUSION: Green-synthesized of TiO2 nanoparticles provides a promising approach to the use of O. vulgare and H. perforatum, carvacrol and hypericin as novel agents and safer antibacterial compounds, especially anti-S. aureus compounds. (C) 2022 JTCM. All rights reserved