Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Tereza Vojtěchová Supervisor: assoc. prof. PharmDr. Iva Boušová, Ph.D. Advisor: PharmDr. Martin Ambrož, Ph.D. Title of diploma thesis: Optimization of liver spheroids preparation Various in vitro liver models play an irreplaceable role in preclinical drug evaluation. One of them is multicellular hepatic spheroids. These are 3D cell aggregates that are formed by the process of cellular aggregation. They are used in drug development and represent a promising tool for identifying new targets and drug screening for various liver diseases. The aim of this thesis was to introduce and optimize the preparation of liver spheroids by the "forced...
In these studies the ability of a three-dimensional hepatocyte-stellate cell co-culture system to pr...
Subject headings liver cell; cell isolation; tissue culure; aggregates AIM To explore a simplif...
Objective To optimize conditions for hypothermic preservation of rat hepatocyte spheroids without fr...
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidat...
Freshly harvested primary rat hepatocytes cultivated as multicellular aggregates, or spheroids, ha...
From PubMed via Jisc Publications RouterPublication status: ppublishFunder: BBSRC; Grant(s): BB/M503...
1 Abstract Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and T...
The liver is a key organ in drug bioavailability including uptake, metabolism, and excretion as well...
Many in vitro liver cell models, such as 2D systems, that are used to assess the hepatotoxic potenti...
To explore peroxisome proliferator-perturbed hepatocyte growth regulation, robust in vitro models of...
Many in vitro liver cell models, such as 2D systems, that are used to assess the hepatotoxic potenti...
Herein, we describe a protocol for the preparation and analysis of primary isolated rat hepatocytes ...
The liver is the most important site of drug metabolism in the human body. During drug development, ...
More predictive in vitro liver models are a critical requirement for preclinical screening of compou...
More predictive in vitro liver models are a critical requirement for preclinical screening of compou...
In these studies the ability of a three-dimensional hepatocyte-stellate cell co-culture system to pr...
Subject headings liver cell; cell isolation; tissue culure; aggregates AIM To explore a simplif...
Objective To optimize conditions for hypothermic preservation of rat hepatocyte spheroids without fr...
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidat...
Freshly harvested primary rat hepatocytes cultivated as multicellular aggregates, or spheroids, ha...
From PubMed via Jisc Publications RouterPublication status: ppublishFunder: BBSRC; Grant(s): BB/M503...
1 Abstract Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and T...
The liver is a key organ in drug bioavailability including uptake, metabolism, and excretion as well...
Many in vitro liver cell models, such as 2D systems, that are used to assess the hepatotoxic potenti...
To explore peroxisome proliferator-perturbed hepatocyte growth regulation, robust in vitro models of...
Many in vitro liver cell models, such as 2D systems, that are used to assess the hepatotoxic potenti...
Herein, we describe a protocol for the preparation and analysis of primary isolated rat hepatocytes ...
The liver is the most important site of drug metabolism in the human body. During drug development, ...
More predictive in vitro liver models are a critical requirement for preclinical screening of compou...
More predictive in vitro liver models are a critical requirement for preclinical screening of compou...
In these studies the ability of a three-dimensional hepatocyte-stellate cell co-culture system to pr...
Subject headings liver cell; cell isolation; tissue culure; aggregates AIM To explore a simplif...
Objective To optimize conditions for hypothermic preservation of rat hepatocyte spheroids without fr...