The fate of phosphorus in root wastewater treatment plants

This bachelor's thesis deals with the determination of phosphorus in constructed wetlands. Phosphorus in wastewater must be eliminated effectively because there is a government regulation that permits maximal permissible concentration of phosphorus on the exit of wastewater treatment plants. Increased concentration in water leads to eutrophication, which causes the death of aquatic animals and plants. The aim of this bachelor's thesis is the validation of an easy and functional method for the determination of phosphorus in the water matrix. This method uses a complex with malachite green. The next aim was the determination of phosphate in the samples from model constructed wetlands. It's possible to make conclusions about the efficiency of model constructed wetlands out of this. For the determination UV-VIS spectrophotometry was used. The measured absorbance was recalculated to a concentration of phosphorus and after that to the concentration of phosphate. During the validation calibration, accuracy measurement, repeatability measurement, and measurement of absorbance dependence on time were performed. Sensitivity (by comparing the malachite green method with the normed method using phosphomolybdate blue), linearity, trueness, robustness, the limit of detection, and the limit of quantification were..