Preparation of fluorescent phthalocyanines for labeling of oligonucleotide probes IV.

Charles University, Faculty of Pharmacy in Hradec Králové Department Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate Daniel Mikula Supervisor doc. PharmDr. Miroslav Miletín, Ph.D. Consultant Mgr. Michaela Kolářová Title of thesis Preparation of fluorescent phthalocyanines for labeling of oligonucleotide probes IV. Phthalocyanines (Pcs) are macrocyclic compounds with a conjugated system of double bonds. Thanks to this, they have interesting photophysical and photochemical properties that enable their practical use in pharmacy and medicine in the field of diagnostics or treatment of cancer diseases. This work deals with the preparation of axially disubstituted silicon complexes of phthalocyanines (SiPcs), which would have suitable properties for subsequent use in the labeling of oligonucleotide probes. The theoretical part is primarily devoted to the methods of Pcs preparation, from the choice of precursors, through the formation of the macrocycle and insertion of the central atom, to the modifications of the resulting molecule, including the specifics related to SiPcs. The basic properties of Pcs and the degree of influence of different changes of the molecule on these properties are also described. The basic areas of use of Pcs are also mentioned. The experimental part..