CUT-AND-RUN DATA ANALYSIS: GENE EXPRESSION DISCOVERY IN CILIOPATHY MICE

Cleavage under targets and release using nuclease coupled with sequencing (CUT&RUN sequencing) is a new technology to detect chromatin-associated protein binding sites and study gene regulation. This study uses Gli3-flagged CUT&RUN data to explore the difference in regulatory landscape between the wild-type and ciliopathy mutant mice in the forelimb and branchial arch tissues. Reduced binding of the GLI family is observed in wild-type mice in both tissues through motif analysis. Some suppressed genes may be reactivated in mutant mice, but binding levels in Gli genes were similar between the two genetic backgrounds