The use amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos

The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos