Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been established as powerful and tunable tools for biospecific applications, be it therapeutics, drug delivery systems or biosensors. It is now generally acknowledged that in vitro selection enables one to generate aptamers to almost any target of interest. However, the success of selection and the affinity of the resulting aptamers depend to a large extent on the nature and design of an initial random nucleic acid library. In this review, we summarize and discuss the most important features of the design of nucleic acid libraries for in vitro selection such as the nature of the library (DNA, RNA or modified nucleotides), the length of a randomized...
We describe the integration of microfluidic selection with high-throughput DNA sequencing technology...
Aptamers are a class of nucleic acid molecules that mimic antibodies by folding, in a sequence speci...
Nucleic acids based therapeutic approaches have gained significant interest in recent years towards ...
Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been e...
Nucleic acid aptamers are single-stranded DNA or RNA oligonucleotide sequences that bind to a specif...
Aptamers are short sequences of single-stranded DNA or RNA that are able to specifically bind variou...
Nucleic acid aptamers are novel molecular recognition tools that offer many advantages compared to t...
Aptamers are an emerging class of molecules that are valued for their ability to bind with high affi...
<p>(A) The sequence of the RNA pool for <i>in vitro</i> selection is shown. The random RNA library w...
In vitro selection and structure-based searches have emerged as useful techniques for the discoverie...
It was only relatively recently discovered that nucleic acids participate in a variety of biological...
In the past five decades, several landmark discoveries have demonstrated that nucleic acids are a mu...
The <i>in vitro</i> selection of nucleic acid libraries has driven the discovery of RNA and DNA rece...
Design of a nucleic acid library is an essential foundation for an in vitro selection. Increasing th...
Preparation of a random‐sequence DNA pool is presented. The degree of randomization and the length o...
We describe the integration of microfluidic selection with high-throughput DNA sequencing technology...
Aptamers are a class of nucleic acid molecules that mimic antibodies by folding, in a sequence speci...
Nucleic acids based therapeutic approaches have gained significant interest in recent years towards ...
Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been e...
Nucleic acid aptamers are single-stranded DNA or RNA oligonucleotide sequences that bind to a specif...
Aptamers are short sequences of single-stranded DNA or RNA that are able to specifically bind variou...
Nucleic acid aptamers are novel molecular recognition tools that offer many advantages compared to t...
Aptamers are an emerging class of molecules that are valued for their ability to bind with high affi...
<p>(A) The sequence of the RNA pool for <i>in vitro</i> selection is shown. The random RNA library w...
In vitro selection and structure-based searches have emerged as useful techniques for the discoverie...
It was only relatively recently discovered that nucleic acids participate in a variety of biological...
In the past five decades, several landmark discoveries have demonstrated that nucleic acids are a mu...
The <i>in vitro</i> selection of nucleic acid libraries has driven the discovery of RNA and DNA rece...
Design of a nucleic acid library is an essential foundation for an in vitro selection. Increasing th...
Preparation of a random‐sequence DNA pool is presented. The degree of randomization and the length o...
We describe the integration of microfluidic selection with high-throughput DNA sequencing technology...
Aptamers are a class of nucleic acid molecules that mimic antibodies by folding, in a sequence speci...
Nucleic acids based therapeutic approaches have gained significant interest in recent years towards ...