Analysis of flavonoids in Achillea nobilis L. bv capillarv electrophoresis

The separation and quantification of the flavonoids occurring in Achillea nobilis L. by a CE-method is described. Using 20 mM sodium borate at pH 9.5 with 20 % of methanol as buffer, the flavonoids were sufficiently separated within 11 minutes. Kaempferol-7-O-neohesperidoside was used as internal standard for the quantification. Analysis of six different samples showed the flavon-C-glycosides isoorientin, orientin and vitexin as the characteristic main compounds. The contents of flavon-C-glycosides determined by this CE-method correlated with the results achieved by a spectrophotometric determination