Photobleaching effects in organic thin film sensing probes

The phenomenon of photobleaching of fluorescence indicators in organic thin films is presented. The study is performed on the fluorescein methacrylate indicator immobilized in an acrylate matrix. A LED based interrogation setup has been realized to evaluate the effects of photobleaching. The experimental results show as stability and drift of the fluorescence signal induced by photobleaching are the most critical aspects in sensing applications