BMP-7 effects on osteogenic differentiation of stem cells

Objectives: To evaluate in preclinical models (in vitro and inanimal model) all requests of Good Manufacturing Practice’srules before the clinical application of mesenchymal stem cells(MSC), in order to elaborate the protocols for using humanautologous MSC in oral regenerative and maxillofacial medicine.Methods: MSC lies in bone marrow an other tissues; they areautologous cells that have not immunological reaction and areexpandable in vitro. MSC are taken from bone marrow samplefrom healthy donor. After medium procedures, an omogeneousSMC colony is reached and its osteoblast differenziating capacitywas tested with or without bone morphogenetic protein-7 (BMP-7). Four medium were used (standard medium, growth medium,osteogenic medium, osteogenic medium with BMP-7).Results: The expression of osteogenic genes are analized bypolimerase chain reaction (PCR): alkaline phosphatase (ALP),osteopontin (SPP1), osteocalcin (BGLAP). In the osteogenicmedium with BMP-7, it was observed an increase of ALP,involved in the early phase of the osteogenetic differenziation,as well as an increase of SPP1 and BGLAP, involved in the latephase of osteogenetic differenziation and mineralization.Conclusions: Results suggest that BMP-7 amplifies the osteogenicdifferenziation process. These results are present in thequality, efficency and safety protocol: “maxillo-facial surgerybone defects by using autologous stem cells expansed in vitro