Predicting Ross River virus infection by analysis of seroprevalence data

Infection with arthropod-borne (arbo)viruses presents a significant and growing public health threat to the resident population of Queensland (QLD), the north-eastern state of Australia. Clinical infection with Ross River virus (RRV) is the most commonly detected, and arguably most debilitating, of Australia’s 75 known indigenous arbovirus species. Development of prediction models to forecast arbovirus epidemics aims to provide accurate and reliable tools that may facilitate planned interventions by local and state authorities to curb disease transmission. Acute immunoglobulin (Ig)M-positive enzyme-linked immunosorbent assay results are often misleading, with interpretation cautioned. As such, this serological testing was recently excluded as a means to confirm cases of arbovirus infection in Australia. The purpose of this study was to investigate the seroepidemiological value of acute IgM-positive results across QLD by correlating with RRV case reports and to develop a mathematical model to predict RRV outbreaks. Blood samples from patients throughout QLD suspected of arboviral infection were tested for RRV, with numbers for various serology results grouped by geographical region. The serology data were compared with case reports for each respective region by multiple regression in order to determine any relationships. RRV IgM-positive results correlated significantly to the number of case reports per region (P 0.05). Hence, these findings failed to validate the potential use of IgM-positive seroprevalence to predict RRV infection with sufficient accuracy for diagnostic purposes. A possible indirect value may exist, however, in analysing pooled seroprevalence data, which may better inform concurrent surveillance measures and thereby enhance the accuracy of RRV outbreak forecasts