Comparative study of assay of free radical damage in in vivo, in vitro and ex vivo conditions

Background: Interception of free radical events and damage in clinical situation remain arbitrary with poor understanding on antioxidant pharmacology. Methods: Experiments on in vitro per-oxidation insult as well as assumed free radical damage situation in ischemia reperfusion are examined for profile of malondialdehyde rise and effect of Gikgobiloba treatment by different schedule.Results: Effect of Ginkgobiloba was seen on ischemia reperfusion induced, hydrogen peroxide induced and ferric chloride induced methods. Antioxidant effect of Ginkgobiloba was consistent in all techniques.Conclusions: Results reveal the significance of complementary experiment to elaborate issue of antioxidants