Random amplified polymorphic DNA (RAPD) analysis for the genetic characterization of potential parents in a breeding program on pot Alstroemeria

Random amplified polymorphic DNA (RAPD) markers were used for the characterization of potential parents in a breeding program on Alstroemeria for pot plant production. A similarity matrix was constructed to estimate the genetic similarity values between genotypes. Cluster analysis was performed to examine the relationships among genotypes. The classification of genotypes through the use of four selected primers is in agreement with the expectations based on geographical origin and pedigree data. On the basis of RAPD data, the most distantly related genotypes were selected to start the breeding program. RAPD analysis thus resulted an effective tool for the genetic characterization of potential parents that may enable ornamental plant breeders to improve the efficiency of the breeding program