TNF-α and IL-10 levels in hipoxic ischemic rat brain receiving stem cell

To investigate the effect of intracerebral adipose neural stem cell transplantation in the inflammatory process after brain HI. The study used 16male Wistar rats aged 2 months which were randomly divided into the following group: Ischemia-Reperfusion group (IR, n=8) and ANSC-treated group (IR+ANSC, n=8). IR was performed by right common carotid artery occlusion for 2 hours, hypoxia procedure for 1 hour and reperfusion.  ANSC was prepared from rats adipose tissue and processed in Laboratory. ANSC was inserted intracerebally after hypoxia procedure and after 48 hours the blood sample were analyzed by using ELISA. T-test for independent samples was used for statistial analysis. There were no significant differences in the TNF-α level between the IR group and IR+ANSC group. There were statistically significant difference in IL-10 level between groups (P< 0.05). Intracerebral ANSC transplantation cannot reduce TNF-α expression and increase IL-10 expression in brain ischemia induced by CCA ligation and hypoxia